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将大鳞大麻哈鱼的垂体细胞特异性表达的生长激素基因（ＧＨ）的启动子与白喉毒素基因的Ａ链相连，构建成融合基因ＧＨ－ＤＴＡ。通过显微注射导入金鱼（Ｃａｒａｓｉｕｓａｕｒａｔｕｓ）受精卵内，获得的实验鱼经Ｓｏｕｔｈｅｒｎｂｌｏｔ分子杂交检测表明，少数实验鱼染色体ＤＮＡ中含有导入的外源基因。胚胎的原位分子杂交和垂体的组织切片观察发现，转基因鱼和正常鱼之间存在差异。
The promoter of growth hormone gene (GH), which is specifically expressed by pituitary cells, was linked to the A chain of diphtheria toxin gene and constructed into fusion gene GH-DTA. The experimental fish obtained by microinjection into Carasiusauratus zygotes detected by Southern blot analysis showed that a few experimental fish chromosomal DNA contained the introduced foreign genes. In situ hybridization of embryos and observation of pituitary tissue sections revealed differences between transgenic fish and normal fish.