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在人工授粉条件下应用明视野显微镜、荧光显微镜和扫描电镜观察了胡杨与小叶杨派间杂交不亲和性的障碍,结果如下: Ⅰ.胡杨×小叶杨:小叶杨的花粉管多数能够顺利地进入胡杨柱头;其中一些花粉管能够正常地在花柱中生长,另一些则因先端沉积胼胝质而停止生长;当花粉管进入子房腔时胚囊尚未成熟,花粉管未能及时进入胚囊。及至胚囊成熟,花粉管衰退,丧失其进入胚囊的能力,故不能发生受精作用。Ⅱ.小叶杨×胡杨:大多数胡杨的花粉管在小叶杨的柱头上呈扭曲、盘绕或爬行状,而且管中往往充满胼胝质,不能穿入柱头;有些柱头细胞有胼胝质反应。少数胡杨的花粉管能够径直地或几经曲折后进入小叶杨的柱头组织中。在花粉管穿经花柱、进入子房腔和到达胚囊的各个阶段都可能因管端沉积胼胝质而停止生长,但也有一些能够正常地进入胚囊,并发生双受精作用。胚大多在球形期或心形期败育,只有少数可发育至成熟。
Under the conditions of artificial pollination, bright field microscopy, fluorescence microscopy and scanning electron microscopy were used to observe the incompatibility barriers between Populus euphratica and Populus tomentosa. The results are as follows: Ⅰ. Populus euphratica × Populus euphratica pollen tube can smoothly Some of the pollen tubes can grow normally in the style while others stop growing due to apex deposition of callose. When the pollen tube enters into the ovary cavity, the embryo sac is not yet mature and the pollen tube fails to enter the embryo sac in time. And until the embryo sac mature, pollen tube decline, the loss of its ability to enter the embryo sac, it can not occur fertilization. Ⅱ. Populus euphratica × Populus euphratica: The pollen tube of most Populus euphratica is twisted, coiled or crawled on the stigma of Populus simonii, and the tube is often filled with callose and can not penetrate the stigma; some stigma cells have callose reaction. A small number of Populus euphratica pollen tube can be straight or after twists and turns into poplar young stigma tissue. In the pollen tube through the style, into the ovary cavity and reach the embryo sac of the various stages may be due to deposition of callose end of the tube to stop growth, but some can enter the normal embryo sac, and double fertilization. Most of the embryo abortion in the spherical or cardiac phase, only a few can be developed to mature.