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探讨茴香霉素对Jurkat T细胞活化、增殖、周期及凋亡的影响。应用双荧光抗体标记结合流式细胞仪检测茴香霉素对豆蔻酰佛波醇乙酯(PMA)和离子霉素(Ion)诱导Jurkat T细胞表面分子CD69和CD25的表达;利用倒置显微镜和噻唑蓝(MTT)比色法观察茴香霉素对Jurkat T细胞的集落形成和增殖影响;采用碘化丙锭(PI)染色检测茴香霉素对Jurkat T细胞周期的影响;应用Annexin V-FITC和PI双染色检测茴香霉素对Jurkat T细胞凋亡的影响。结果表明,在PMA和Ion共同刺激下随茴香霉素剂量上升CD69和CD25的表达量逐渐下降;随着茴香霉素剂量增高,Jurkat T细胞的集落形成减小,其对细胞增殖的抑制率最高可达74.29%,使细胞周期停滞于G0/G1期之前;当茴香霉素剂量为40.0~160.0 ng/ml时能明显促进Jutkat T细胞凋亡。结果提示,茴香霉素能明显抑制PMA和Ion刺激下的Jurkat T细胞早期和中期活化,抑制Jurkat T细胞的增殖,使细胞周期停滞于G0/G1期之前,并能促进Jurkat T细胞凋亡。
To investigate the effect of anisomycin on activation, proliferation, cycle and apoptosis of Jurkat T cells. Double immunofluorescent labeling and flow cytometry were used to detect the expression of CD69 and CD25 on Jurkat T cells induced by anisomycin (PMA) and ionomycin (Ion). Using inverted microscope and thiazole blue (MTT) assay was used to observe the effect of anisomycin on Jurkat T cell colony formation and proliferation. The effect of anisomycin on Jurkat T cell cycle was detected by propidium iodide (PI) staining. Annexin V-FITC and PI bis Effect of Anisomycin on Apoptosis of Jurkat T Cells Detected by Staining. The results showed that under the stimulation of PMA and Ion, the expression of CD69 and CD25 decreased gradually with the increase of dose of anisomycin. With the increase of dose of anisomycin, the Jurkat T cell colony formation was reduced and the inhibition rate of cell proliferation was the highest Up to 74.29%, the cell cycle arrest before G0 / G1 phase; when the dose of anisomycin 40.0 ~ 160.0 ng / ml can significantly promote apoptosis of Jutkat T cells. The results suggest that anisomycin can significantly inhibit the early and mid-term activation of Jurkat T cells stimulated by PMA and Ion, inhibit the proliferation of Jurkat T cells, arrest the cell cycle before G0 / G1 phase and promote the apoptosis of Jurkat T cells.