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本研究探索自组装短肽GFS-4在心肌细胞三维培养中的应用效果及其对心肌梗死区域的组织修复作用。通过圆二色谱仪分析短肽GFS-4的二级结构,原子力显微镜检测短肽GFS-4自组装的微观形态。将GFS-4自组装形成的纳米纤维支架作为心肌细胞三维培养材料,观察心肌细胞的生长状况;建立大鼠心肌梗死模型,加入水凝胶GFS-4研究其对心肌梗死修复的效果。结果发现,GFS-4自组装形成的二级结构主要为β折叠;自组装24 h后形成致密的纳米纤维支架;心肌细胞三维培养结果表明心肌细胞在GFS-4水凝胶中生长状况良好;心肌梗死体外修复实验发现,短肽GFS-4水凝胶支架可缓解心肌梗死区域组织坏死。自组装短肽GFS-4作为新的纳米支架材料,可用于细胞三维培养和心肌梗死区域组织修复。
This study explored the application of self-assembled short peptide GFS-4 in cardiomyocyte three-dimensional culture and its effect on tissue repair in myocardial infarction area. The secondary structure of the short peptide GFS-4 was analyzed by circular dichroism spectroscopy. The microscopic morphology of GFS-4 self-assembled peptide was detected by atomic force microscopy. The self-assembled nanofibrous scaffolds with GFS-4 were used as the three-dimensional culture material of cardiomyocytes to observe the growth of cardiomyocytes. The model of myocardial infarction in rats was established. The effect of GFS-4 hydrogel on myocardial infarction was studied. The results showed that the secondary structure formed by GFS-4 self-assembly was β-sheet. The dense nanofibrous scaffolds formed after self-assembly for 24 h. The three-dimensional culture of cardiomyocytes indicated that the myocytes grew well in GFS-4 hydrogel. Myocardial infarction in vitro repair experiments found that short peptide GFS-4 hydrogel scaffold can ease myocardial infarction area tissue necrosis. Self-assembled short peptide GFS-4 as a new nano-scaffold material can be used for three-dimensional cell culture and regional tissue repair of myocardial infarction.