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水稻脆性突变体是研究细胞壁组分结构形成机制的重要材料。通过离子束诱变籼稻9311获得1个茎秆、叶片均脆的突变体,命名为bc9311-1。bc9311-1突变体与野生型9311相比,分蘖数减少,结实率显著降低,其他农艺性状无明显差异。叶片和茎秆的细胞壁成分分析表明,与野生型相比,bc9311-1突变体茎秆中的纤维素和木质素含量明显降低,半纤维素和SiO2含量显著增加;叶片中的纤维素含量降低,半纤维素和木质素含量增加,SiO2含量无明显差异。遗传分析表明,该脆性突变体脆性性状受单隐性基因控制。以bc9311-1突变体与02428杂交的F2群体为基因定位群体,利用SSR标记将bc9311-1突变位点定位在水稻第1染色体上,位于SSR分子标记的RM1095和RM3632之间,遗传距离分别为0.6cM和3.4cM,与其中的标记RM1183表现共分离。这些结果为进一步克隆突变基因,揭示脆性性状的分子机制奠定坚实基础。
Rice brittle mutants are important materials for studying the formation mechanism of cell wall components. A mutant with stalks and leaves were obtained by ion beam mutagenesis of indica rice 9311 and named as bc9311-1. Compared with the wild-type 9311, the number of tillers decreased and the seed setting rate of bc9311-1 mutant decreased significantly, while the other agronomic traits showed no significant difference. Analysis of the cell wall composition of the leaves and stems showed that the content of cellulose and lignin in the bc9311-1 mutant stalk was significantly decreased and the content of hemicellulose and SiO2 was significantly increased compared with the wild type; the cellulose content in the leaves was decreased , Hemicellulose and lignin content increased, SiO2 content no significant difference. Genetic analysis showed that the brittle traits of brittle mutants were controlled by a single recessive gene. The bc9311-1 mutant and F2 population crossed with 02428 were used as genetic loci. The site of bc9311-1 mutation was located on rice chromosome 1 using SSR markers and located between SSR markers RM1095 and RM3632. The genetic distances were 0.6cM and 3.4cM, which co-segregation with the marker RM1183 performance. These results lay a solid foundation for further cloning mutant genes and revealing the molecular mechanism of brittle traits.