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目的:探讨Runx3基因启动子甲基化与喉癌发生发展过程的关系。方法:采用RT-PCR检测40例喉癌组织及肿瘤周围黏膜组织中Runx3 mRNA的表达,同时采用甲基化特异性PCR(MSP)方法检测Runx3基因启动子甲基化情况。结果:40例喉癌组织标本中Runx3基因的表达(1.62±1.01)较肿瘤周围组织中表达(5.66±2.07)明显下调,差异有统计学意义(P<0.01)。正常黏膜组织中未发现有Runx3基因启动子的甲基化,40例喉癌组织中有38例检测到Runx3基因启动子的甲基化,喉癌组织Runx3基因启动子甲基化率显著增高(P<0.01)。喉癌标本中Runx3 mRNA的表达与其病理临床特征密切相关,在低分化和有淋巴结转移喉癌组织标本中Runx3 mRNA的表达显著下调(P<0.01)。结论:Runx3基因启动子甲基化是导致Runx3基因失活的主要原因之一并且与喉癌的发生、发展密切相关。
Objective: To investigate the relationship between Runx3 promoter methylation and laryngeal carcinogenesis. Methods: The expressions of Runx3 mRNA in 40 cases of laryngeal carcinoma and surrounding mucosa tissues were detected by RT-PCR. Methylation-specific PCR (MSP) was used to detect the promoter methylation of Runx3 gene. Results: The expression of Runx3 gene in 40 specimens of laryngeal carcinoma (1.62 ± 1.01) was significantly lower than that in the surrounding tissues (5.66 ± 2.07), the difference was statistically significant (P <0.01). No methylation of Runx3 promoter was found in normal mucosa tissues, methylation of Runx3 promoter was detected in 38 of 40 laryngeal cancer tissues, and Runx3 promoter methylation rate was significantly increased in laryngeal carcinoma ( P <0.01). The expression of Runx3 mRNA in laryngeal cancer specimens is closely related to its clinical features. The expression of Runx3 mRNA in laryngeal cancer tissues with poorly differentiated and lymph node metastasis was significantly down-regulated (P <0.01). Conclusion: Runx3 promoter methylation is one of the major causes of Runx3 gene inactivation and is closely related to the occurrence and development of laryngeal carcinoma.