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为研究砷对大鼠脑组织氨基酸神经递质水平的影响并探讨砷的脑损伤机制,将SD大鼠(雄雌各半)随机分为对照组(A组)、子代染砷组(B组)、母子染砷组(C组)。A、B组饮蒸馏水,C组饮As2O3水溶液[50mg/L,相当于10mg/(kg·d)],6周时,各组雌雄分别2:1合笼产仔,仔鼠乳养至断乳,仔鼠继续按本组剂量染砷到断乳后第12周。测定仔鼠的大脑皮质氨基酸(天冬氨酸、谷氨酸、赖氨酸、甘氨酸、γ-氨基丁酸、精氨酸、丙氨酸、组氨酸)水平。各组仔鼠分别于断乳后染砷0、4、12和24周时称取体重。结果显示,砷暴露12周的B组和C组的仔鼠大脑皮层谷氨酸和天冬氨酸水平均高于对照组,以C组的仔鼠谷氨酸升高更明显,差异有统计学意义(P<0.01),r-氨基丁酸、甘氨酸等与对照组比较无显著差异。B组雄性仔鼠染砷4和12周时的体重增长均高于对照组(P<0.01),而24周时B组的雄性仔鼠体重增长则明显低于对照组(P<0.01);B组雌性仔鼠前4周体重增长高于对照组(P<0.01),12周和24周时体重增长则明显低于对照组;C组的雄、雌仔鼠体重增长均低于对照组和B组的雄、雌仔鼠(P<0.01)。本研究的砷暴露剂量能致子代大鼠大脑皮层谷氨酸水平升高,以母代染砷的仔鼠升高更显著。砷暴露初期有促进大鼠体重增长效应,长期砷暴露对体重增长幅度有抑制作用,以母代染砷的仔鼠更显著且雌性仔鼠更敏感。
In order to study the effect of arsenic on amino acid neurotransmitter in rat brain and explore the mechanism of arsenic brain damage, SD rats (male and female half) were randomly divided into control group (group A) Group), mother and child stained arsenic group (C group). A, B group drinking distilled water, C group drinking As2O3 aqueous solution [50mg / L, equivalent to 10mg / (kg · d)], 6 weeks, each group of male and female were 2: 1 cage farrowing, Milk, offspring continue to dose according to this group stained arsenic to 12 weeks after weaning. The level of amino acids (aspartic acid, glutamic acid, lysine, glycine, gamma-aminobutyric acid, arginine, alanine, histidine) in the cerebral cortex of offspring was determined. The pups were weighed at 0, 4, 12 and 24 weeks after weaning respectively. The results showed that the levels of glutamate and aspartate in the cerebral cortex of group B and C were higher than those of the control group at 12 weeks of arsenic exposure, and the increase of glutamate in group C was more obvious Significance (P <0.01), r-aminobutyric acid, glycine compared with the control group no significant difference. The weights of male offspring in group B were higher than those in control group at 4 and 12 weeks of arsenic exposure (P <0.01), while the weight gain of male offspring in group B at 24 weeks was significantly lower than that of control group (P <0.01). The weight gain in the first 4 weeks of female offspring of group B was higher than that of control group (P <0.01), and the weight gain of 12 weeks and 24 weeks was lower than that of control group. The weight gain of male and female offspring of group C was lower than that of control group And male and female rats in group B (P <0.01). The arsenic exposure dose in this study can lead to elevated levels of glutamate in the cerebral cortex of progeny rats, and more significant increases in offspring of arsenic-bearing offspring. In the early stage of arsenic exposure, it can promote weight gain in rats. Long-term exposure to arsenic has an inhibitory effect on weight gain. More offspring are arsenic-bearing offspring and more sensitive to female offspring.