论文部分内容阅读
目的了解深圳地区肾综合征出血热疫点鼠形动物汉坦病毒的带毒情况和抗体水平,为本市肾综合征出血热的预防和控制提供科学依据。方法用酶联免疫吸附(ELISA)法检测鼠血清汉坦病毒Ig M抗体和Ig G抗体,并用汉坦病毒定量试剂盒测定汉坦病毒总抗体浓度。结果深圳地区肾综合征出血热疫点的鼠种主要为褐家鼠(占50.82%)和臭鼩鼱(占47.51%)。鼠类汉坦病毒Ig M抗体和Ig G抗体阳性率分别为10.5%和16.6%。鼠形动物血清汉坦病毒总抗体浓度为(54.98±9.87)pg/ml。结论深圳地区褐家鼠具有较高的汉坦病毒感染率和抗体水平,同时臭鼩鼱在疫点鼠中的比例较大,并可携带汉坦病毒。应加强肾综合征出血热疫点的灭鼠和后续监测工作。
Objective To understand the virulence status and antibody level of Hantavirus in mice with HFRS in Shenzhen and to provide a scientific basis for the prevention and control of HSH. Methods The serum anti-Hantavirus Ig M and Ig G antibodies were detected by enzyme-linked immunosorbent assay (ELISA). The total Hantaan virus concentration was determined by Hantavirus Quantitative Kit. Results The main species of RNSH in Shenzhen were Rattus norvegicus (50.82%) and Smecta (47.51%). The positive rates of murine Hantavirus Ig M and Ig G antibodies were 10.5% and 16.6%, respectively. The concentration of total Hantavirus in murine serum was (54.98 ± 9.87) pg / ml. Conclusion Rattus norvegicus in Shenzhen has higher infection rate and antibody level of Hantavirus, and the proportion of Stenotrophomonas japonicus in the immunodeficient rats is larger than that of Hantavirus in Shenzhen. Rodent control and follow-up monitoring of hemorrhagic fever with renal syndrome should be strengthened.