用悬浮聚合法制备了六批磁性微珠，其表面有相同量的胺基功能基团；用戊二醛一步法，对αＨＣＧ单抗标记了碱性磷酸酶，用βＨＣＧ单抗制备了磁珠一抗，测得ＨＣＧ浓度为５００ｍＵ／ｍｌ、２５０ｍＵ／ｍｌ、１００ｍＵ／ｍｌ时的ＯＤ值ＣＶ偏差分别为４６％、６８％和７０％。 Six batches of magnetic beads were prepared by suspension polymerization with the same amount of amine-functional groups on their surface. One-step glutaraldehyde method was used to label alkaline phosphatase (α-HCG) with α-HCG monoclonal antibody The primary antibodies were prepared. The CV deviations of OD values ​​of HCG at 500mU / ml, 250mU / ml and 100mU / ml were 46%, 68% and 70%, respectively.