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目的建立酸性胃食管反流性疾病(GERD)的豚鼠模型,用于研究胃食管反流性疾病并发的呼吸系统疾病。方法豚鼠持续灌酸或蒸馏水16天后,测其食管下段粘膜的pH值,计数支气管肺泡灌洗液(BALF)中的细胞总数及嗜酸性粒细胞计数,并研取食管、气管和肺组织标本进行病理学观察。结果模型组豚鼠食管下段粘膜的pH值明显降低,BALF的细胞总数和嗜酸性粒细胞(EOS)显著升高(P<0.01),病理变化主要表现为食管、气管和肺组织上皮增生,炎性细胞和嗜酸性粒细胞的浸润。结论胃内容物反流能造成豚鼠食管、气管粘膜和肺组织的损伤,可研究GERD并发的呼吸系统疾病和该病的病理生理情况,该制模方法成活率高、简单易操作。
Objective To establish a guinea pig model of acidic gastroesophageal reflux disease (GERD) for the study of respiratory diseases associated with gastroesophageal reflux disease. Methods Guinea pigs were drenched with acid or distilled water continuously for 16 days. The mucosal pH value of lower esophageal mucosa was measured. The total number of cells and eosinophil count in bronchoalveolar lavage fluid (BALF) were counted. The esophagus, trachea and lung tissue samples Pathological observation. Results The pH value of mucosa in the lower esophageal mucosa of guinea pigs was significantly decreased, the total number of cells and eosinophils (EOS) in BALF were significantly increased (P <0.01), and the pathological changes were mainly epithelial hyperplasia and inflammatory in esophagus, trachea and lung tissue Infiltration of cells and eosinophils. Conclusions The reflux of gastric contents can cause the damage of esophagus, tracheal mucosa and lung tissue in guinea pigs. It can be used to study the respiratory diseases and the pathophysiology of GERD. The method has the advantages of high survival rate, simple and easy operation.