论文部分内容阅读
目的探讨系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中寡腺苷酸合成酶(OAS)1、OAS2和OASLmRNA的实时定量表达水平与SLE疾病特异性和病情活动度的相关性。方法收集144例SLE患者、27例非SLE患者与59名正常对照人群的临床资料,取外周血抽取总RNA并反转录成cDNA,运用sybrgreendyeⅠ实时定量聚合酶链反应(PCR)法在ABIPRISM7900H基因测序仪上检测患者组和对照组的OAS1、OAS2的OASLmRNA表达水平的差异,并与病情活动度进行分组比较,分析其意义。结果①SLE患者的总体OAS1mRNA定量表达水平显著高于非SLE对照组和正常对照组,差异有非常显著性(P=0郾016,P=0郾000);OAS2mRNAP定量表达水平高于正常对照组(P=0郾015);而OASLmRNA定量表达水平与非SLE对照组和正常组之间差异无显著性(P=0郾698,P=0郾396)。②SLE活动组的OAS1和OASLmRNA定量表达水平均显著高于SLE非活动组,差异有非常显著性(P=0郾011,P=0郾001)。但SLE活动组的OAS2表达水平与SLE非活动组之间差异无显著性(P=0郾109)。③SLE患者组的OAS2和OASLmRNA定量表达水平与SLEDAI积分呈正相关性(P=0郾001,P=0郾006)。④SLE患者的肾、肺、脑、血液等器官的有无受累与OAS1、OAS2和OASLmRNA表达水平无相关性。⑤SLE患者组和正常对照组组内的OAS1、OAS2和OASL表达水平
Objective To investigate the correlation between real-time quantitative expression of oligoacyl-synthase (OAS) 1, OAS2 and OASL mRNA in peripheral blood mononuclear cells (PBMC) of patients with systemic lupus erythematosus (SLE) and SLE disease-specific and disease activity . Methods The clinical data of 144 patients with SLE, 27 patients with non-SLE and 59 normal control subjects were collected. Total RNA was extracted from peripheral blood and reverse transcribed into cDNA. The sybrid greendye Ⅰ real-time quantitative polymerase chain reaction (PCR) The difference of OASL mRNA expression levels in OAS1 and OAS2 between the patient group and the control group was detected by sequencer and compared with that of the disease activity group to analyze the significance. Results ① The quantitative expression of OAS1 mRNA in patients with SLE was significantly higher than that in non-SLE patients and normal controls (P = 0 郾 016, P = 0 郾 000). The quantitative expression level of OAS2 mRNA was higher than that in the normal controls P = 0 郾 015). However, there was no significant difference between OASL mRNA expression level and non-SLE control group and normal group (P = 0 郾 698, P = 0 郾 396). ② The levels of OAS1 and OASL mRNA in SLE group were significantly higher than those in SLE group (P = 0 郾 011, P = 0 郾 001). However, there was no significant difference between OLE2 expression level and SLE inactive group (P = 0 郾 109). (3) The quantitative expression of OAS2 and OASL mRNA in patients with SLE was positively correlated with the SLEDAI score (P = 0 郾 001, P = 0 郾 006). ④SLE patients with kidney, lung, brain, blood and other organs with or without involvement of OAS1, OAS2 and OASL mRNA expression levels were not related. ⑤SLE patient group and normal control group OAS1, OAS2 and OASL expression levels