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通过对实验动物肺灌洗过程灌洗回收液中总磷脂、二棕榈酰卵磷脂（ＤＰＰＣ）含量测定及灌洗后即刻肺组织光镜、电镜学观察，结果显示：灌洗回收液中总磷脂、ＤＰＰＣ含量随灌洗次数递增而递减，灌洗后即刻肺组织形态学表现为部分肺泡结构轻度紊乱，肺泡腔扩张，肺泡Ⅱ型上皮细胞表面微绒毛稀疏，短粗，细胞内板层体数量减少，呈排空状，提示灌洗可造成肺表面活性物质的丢失。
The content of total phospholipids, dipalmitoyl lecithin (DPPC) in lung lavage fluid of experimental animals after lavage and the light microscope and electron microscopy of lung tissue immediately after lavage were observed. The results showed that total phospholipids , The content of DPPC decreased with the increase of lavage number, the morphology of lung tissue immediately after lavage showed mild disturbance of part of alveolar structure, alveolar dilatation, thinning of microvilli on surface of alveolar type Ⅱ epithelial cells, stubby, intracellular lamina The number decreased, was empty, suggesting that lavage can cause the loss of pulmonary surfactant.