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目的采用染色法判别日本血吸虫尾蚴的生命状态。方法以0.4%台盼蓝、0.5%M.E.B、0.5%伊红、0.5%甲基蓝、0.05%中性红等5种染色液分别对死活日本血吸虫尾蚴染色5 min,显微镜下观察着色情况。结果台盼蓝、M.E.B、伊红、中性红4种染色液可使死尾蚴着色,而甲基蓝染液对其不着色;5种染液均不能使活尾蚴着色。结论0.4%台盼蓝、0.5%M.E.B、0.5%伊红、0.05%中性红染液染色可判别日本血吸虫尾蚴的生命状态。
Objective To determine the state of life of Schistosoma japonicum cercariae by using staining method. Methods The live and inactivated cercariae of Schistosoma japonicum were stained with 0.4% trypan blue, 0.5% M.E.B, 0.5% eosin, 0.5% methyl blue and 0.05% neutral red for 5 min. Results Trypan blue, M.E.B, Eosin, neutral red 4 stains can make dead cercariae stain, while methyl blue dye does not stain them; 5 kinds of dye solution can not live cercariae staining. Conclusion 0.4% trypan blue, 0.5% M.E.B, 0.5% Eosin, 0.05% neutral red dye staining can determine the status of Schistosoma japonicum cercariae.