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目的 :研究持续腹腔冲洗化疗对裸小鼠人卵巢上皮癌腹腔荷瘤模型的疗效及其副作用。方法 :用卵巢浆液性囊腺癌细胞株 Skov3和裸小鼠建立腹腔荷瘤模型 ,分对照组、常规 CDDP腹腔化疗组 (常规组 )和 CDDP腹腔持续冲洗化疗组 (冲洗组 )进行观察。于移瘤后 3w开始治疗 ,间隔 4d,共 2个疗程。 结果 :冲洗组动物的体重在治疗后虽有所下降 ,但远未达到毒性反应的程度 ,与常规组相比 ,无显著差异。冲洗组腹腔瘤的体积明显小于常规组 ,抑瘤率和细胞凋亡率明显增高 ,差异十分显著。病理组织学和超微病理学检查也显示 ,腹腔冲洗化疗效果优于常规化疗 ,对肾脏也没有大的病理损伤。结论 :对裸小鼠腹腔荷瘤 ,持续腹腔冲洗化疗较常规腹腔化疗疗效显著 ,无明显的毒副作用。
Objective: To study the curative effect and side effects of continuous intraperitoneal chemotherapy on nude mice model of human ovarian cancer. Methods: The ovarian serous cystadenocarcinoma cell line Skov3 and nude mice were used to establish the model of peritoneal tumor. The control group, conventional CDDP intraperitoneal chemotherapy group (routine group) and CDDP intraperitoneal continuous chemotherapy group (rinse group) were observed. 3w after tumor metastasis began treatment, an interval of 4d, a total of 2 courses. Results: Although the body weight of the animals in the rinsing group decreased after treatment, they were far from the degree of toxicity, showing no significant difference compared with the conventional group. The volume of abdomen tumor in rinse group was significantly smaller than that in routine group, and the tumor inhibition rate and apoptosis rate were significantly increased, the difference was significant. Histopathological and ultrastructural pathological examination also showed that peritoneal washout chemotherapy is superior to conventional chemotherapy, no significant pathological damage to the kidneys. Conclusion: In nude mice peritoneal tumor, continuous intraperitoneal chemotherapy is more effective than conventional intraperitoneal chemotherapy, no obvious side effects.