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目的建立血清梅毒螺旋体IgG抗体亲和指数(avidity index,AI)测定方法,以探讨区分梅毒近期感染与既往感染的可行性。方法用间接ELISA检测梅毒感染病人血清中的抗梅毒螺旋体IgG抗体,通过尿素处理破坏低结合能力的抗体,计算梅毒螺旋体IgG抗体的AI。结果对照组(c)为107份既往感染者,其AI为(75.3±11.9)%,AI的95%下可信限为51.9%,低于此下限值可诊断为近期感染;观察组(a、b)的AI阳性率分别为53.8%(14/26)、6.7%(3/45);观察组(a)与对照组(c)或观察组(b)比较,差异均有统计学意义(χ2=37.39、17.64,P<0.01);观察组(b)与对照组(c)比较,差异无统计学意义(χ2=0.01,P>0.01)。结论梅毒螺旋体IgG抗体AI可有效区分梅毒近期感染和既往感染。
OBJECTIVE: To establish a method for determining the avidity index (AI) of serum syphilis antibody of Treponema Pallidum to explore the feasibility of distinguishing between recent infection and previous infection of syphilis. Methods The anti-Treponema Pallidum IgG antibody in sera of syphilis-infected patients was detected by indirect ELISA. The AI of the anti-Treponema pallidum IgG antibody was calculated by urea treatment. Results The control group (c) was 107 previously infected patients, with an AI of (75.3 ± 11.9)% and a 95% confidence interval (AI) of 51.9%. A lower than this limit was diagnosed as a recent infection. The positive rates of AI in a and b were 53.8% (14/26) and 6.7% (3/45) respectively. The difference between the observation group (a) and the control group (c) or observation group (b) (Χ2 = 37.39,17.64, P <0.01). The difference between the observation group (b) and the control group (c) was not statistically significant (χ2 = 0.01, P> 0.01). Conclusion Treponema pallidum IgG antibody AI can effectively distinguish the recent infection and the past infection of syphilis.