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目的:建立金线莲中芦丁的含量测定方法。方法:采用高效液相色谱法。选用Lanbo-Kromasil-C18色谱柱(250 mm×4.6 mm,5μm);以甲醇-0.2%磷酸溶液(40∶60)为流动相;检测波长:257 nm,流速:1.0 ml·min-1,柱温:30℃。结果:芦丁在进样量0.021~0.433μg范围内呈良好的线性关系(r=0.999 6)。平均加样回收率为96.65%(RSD=1.67%,n=9)。结论:本法简便、快速、专属性强、重复性好。
Objective: To establish a method for the determination of rutin in Anoectochilus. Methods: Using high performance liquid chromatography. The mobile phase consisted of LanBa-Kromasil-C18 column (250 mm × 4.6 mm, 5 μm) and methanol-0.2% phosphoric acid solution (40:60). The detection wavelength was 257 nm and the flow rate was 1.0 ml · min -1. Temperature: 30 ℃. Results: Routine showed a good linearity (r = 0.999 6) in the range of 0.021-0.433 μg. The average recovery was 96.65% (RSD = 1.67%, n = 9). Conclusion: This method is simple, rapid, specific and reproducible.