CTLL细胞是依赖IL－2生长的细胞株。常用于IL－2生物活性的测定，由于外界环境改变或因各个实验室条件的差异．易造成引进后培养失败。本实验室在饲养该细煦林中总结了几点体会．现报道如下。1材料CTLL细胞株由第四军医大学免疫教研室引进。培养基为含1％G－L和双抗，20mMHepes CTLL cells are IL-2-dependent cell lines. Commonly used in the determination of biological activity of IL-2, due to changes in the external environment or due to different laboratory conditions. Easily lead to failure after the introduction of training. The laboratory in feeding the fine Xu Lin summed up a few experiences. Report as follows now. 1 material CTLL cell line by the Fourth Military Medical University Department of Immunization introduced. The medium consisted of 1% G-L and double antibody, 20 mM Hepes