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目的：研究嘌呤能Ｐ２ｚ受体在介导慢性淋巴细胞白血病（ＣＬＬ）细胞凋亡中的作用。方法：将表达Ｐ２ｚ受体［Ｐ２ｚ（＋）］与不含Ｐ２ｚ受体［Ｐ２ｚ（－）］的两组ＣＬＬ细胞分别同１．０ｍｍｏｌ／Ｌ三磷酸腺苷（ＡＴＰ）体外培养８小时，以电镜、ＤＮＡ凝胶电泳和定量ＤＮＡ３′端ＴｄＴ法检测细胞凋亡；并对ＡＴＰ、苯甲酰苯甲酸ＡＴＰ（ＢｚＡＴＰ）、２甲基硫ＡＴＰ（２ＭｅＳＡＴＰ）、γ硫代ＡＴＰ（ＡＴＰγＳ）及其它核苷的诱导效应和氧化型ＡＴＰ（ＯｘＡＴＰ）、１［Ｎ，Ｏ二（５异喹啉碘酰基）Ｎ甲基Ｌ酪氨酰］４苯哌嗪（ＫＮ６２）的抑制效应做定量研究。结果：①Ｐ２ｚ＋细胞能在ＡＴＰ诱导下呈现典型的细胞凋亡形态和ＤＮＡ梯形条带；②不同的Ｐ２ｚ受体激活剂可通过Ｐ２ｚ受体诱导细胞凋亡并产生不同量的ＤＮＡ降解片段，诱导能力的强弱顺序是ＢｚＡＴＰ＞ＡＴＰ＞２ＭｅＳＡＴＰ，而ＡＴＰγＳ或其它核苷几乎无诱导能力；③ＯｘＡＴＰ和ＫＮ６２可完全阻止诱导剂诱导的细胞凋亡。结论：Ｐ２ｚ受体在介导由ＡＴＰ等诱导ＣＬＬ细胞凋亡的过程中起着十分重要的作用
Objective: To investigate the role of purinergic P2z receptors in mediating apoptosis of chronic lymphocytic leukemia (CLL) cells. METHODS: Two groups of CLL cells expressing P2z receptor [P2z(+)] and P2z receptor-free [P2z(-)] were incubated with 1.0 mmol/L of adenosine triphosphate (ATP) in vitro for 8 hours to electron microscope and DNA. Gel electrophoresis and quantitative DNA 3′-end TdT assay were used to detect cell apoptosis; and ATP, benzoylbenzoic acid ATP (BzATP), 2methylsulfide ATP (2MeSATP), γthio-ATP (ATPγS) and Induction effects of other nucleosides and oxidized ATP (OxATP), 1 [N, O 2 (5 - isoquinoline iodinyl) N methyl L tyrosyl] 4 - phenylpiperazine (KN Quantitative study of the inhibitory effects of 62). RESULTS: 1P2z+ cells could exhibit typical apoptotic morphology and DNA trapezoidal bands when induced by ATP; 2 different P2z receptor activators could induce apoptosis and produce different amounts of DNA degradation fragments through P2z receptors. The order of strength is BzATP>ATP>2MeSATP, while ATP-γS or other nucleosides have almost no induction ability; 3OxATP and KN-62 can completely prevent inducer-induced apoptosis. Conclusion: P2z receptor plays an important role in mediating the apoptosis of CLL cells induced by ATP and so on.