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目的:探讨喉癌前病变及喉鳞状细胞癌病变组织上杂合性缺失(LOH)的特征及其意义。方法:选取染色体3p、9p和17p上6个多态性微卫星位点D3S1234、D9S171、D9S1748、D9S162、INFA和p53,利用聚合酶链式反应-简单序列长度多态性-银染技术,对49例喉癌前病变和喉癌组织进行LOH分析。结果:6个微卫星标记物LOH发生率分别为:单纯过度增生3.70%,轻度不典型增生10.81%,重度不典型增生26.03%,喉鳞状细胞癌38.67%。其中LOH的总检出率在不同病理组间差异有统计学意义(χ2=17.686,P<0.01),其频率随病变程度加重而明显升高。6个多态性微卫星位点中,LOH发生率最高的位点D9S171(35.00%)。结论:基因水平的改变发生在喉癌变的早期阶段,微卫星标志物可能成为喉癌前病变早期诊断的有用标志物。
Objective: To investigate the characteristics and significance of loss of heterozygosity (LOH) in the lesions of laryngeal precancerous lesions and laryngeal squamous cell carcinomas. Methods: Six polymorphic microsatellite loci, D3S1234, D9S171, D9S1748, D9S162, INFA and p53 on chromosomes 3p, 9p and 17p, were selected and analyzed by polymerase chain reaction-simple sequence-length polymorphism 49 cases of laryngeal precancerous lesions and laryngeal tissue LOH analysis. Results: The incidence of LOH in 6 microsatellite markers were 3.70%, 10.81%, 26.03% and 38.67%, respectively. The total detection rate of LOH in different pathological groups was statistically significant difference (χ2 = 17.686, P <0.01), the frequency increased with the severity of the disease was significantly increased. Of the six polymorphic microsatellite loci, D9S171 (35.00%) had the highest incidence of LOH. CONCLUSIONS: Gene level changes occur in the early stage of laryngeal carcinogenesis. Microsatellite markers may be useful markers for the early diagnosis of laryngeal precancerous lesions.