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目的探讨侯氏黑散联合环境干预对脑缺血后神经功能的康复作用。方法采用数字表法将动物随机分为假手术组、模型组、丰富环境组、侯氏黑散组和侯氏黑散联合丰富环境组(联合组)。采用大鼠大脑中动脉闭塞模型复制脑缺血动物模型,通过横木行走实验评价大鼠精细运动功能恢复情况;触觉刺激实验评价躯体感觉和精细运动执行功能;病理形态学检测评价神经细胞的损伤程度。结果联合组大鼠在造模后7 d,横木行走能力、触觉敏感度及精细运动功能均明显恢复,与模型组差异显著。而侯氏黑散组及丰富环境组神经功能恢复明显滞后,在术后12 d后神经功能比模型组明显提高。与模型组相比,联合组与侯氏黑散组皮层、海马CA1区完整锥体细胞数显著增多。联合组动物锥体细胞数明显比侯氏黑散组多。与模型组相比,联合组与侯氏黑散组大鼠神经元变性程度均明显减轻。与模型组相比,联合组与侯氏黑散组神经细胞尼氏体含量均明显提高(P<0.01);联合组与侯氏黑散组2组之间进行比较,没有显著性差异。结论侯氏黑散联合丰富环境对神经细胞的保护作用比单独中药治疗或者仅用环境干预更有优势。
Objective To investigate the effect of Houshiheisan combined with environmental intervention on neurological function after cerebral ischemia. Methods The animals were randomly divided into sham operation group, model group, rich environment group, Houshiheisan group and Houshiheisan combined environment group (combination group) by digital table method. Rat model of middle cerebral artery occlusion was used to replicate the model of cerebral ischemia. The recovery of fine motor function was evaluated by horizontal walking test. Tactile stimulation test was used to evaluate somatic sensation and fine motor performance. Pathological examination was used to assess the damage of nerve cells . Results At 7 days after modeling, the walking ability, tactile sensitivity and fine motor function of the rats in the combined group were significantly recovered, with significant differences from the model group. However, the neurological function recovery of Houshiheisan group and Envrience Enviromental Group lagged significantly, and the neurological function was significantly increased 12 d after operation. Compared with the model group, the number of complete pyramidal neurons in the cortex and hippocampal CA1 region in the combined group and Houshiheisan group increased significantly. The number of pyramidal cells in the combined group was significantly higher than that in the Holstein group. Compared with the model group, the degree of neuron degeneration in the combined group and Houshiheisan group were significantly reduced. Compared with the model group, the content of Nissl in neurons in the combination group and Houshiheisan group were significantly increased (P <0.01); there was no significant difference between the two groups in the combination group and Houshiheisan group. Conclusion The protective effect of Houshiheosan combined with rich environment on nerve cells is superior to the traditional Chinese medicine treatment alone or with environmental intervention alone.