,Propofol attenuates high glucose-induced P66shc expression in human umbilical vein endothelial cell

来源 :生物化学与生物物理学报(英文版) | 被引量 : 0次 | 上传用户:ff303
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Perioperative hyperglycemia is a common metabolic disorder in clinic settings.Hyperglycemia leads to endothelial inflammation,endothelial cell apoptosis,and dysfunction,thus resulting in endothelial injury.Propofol (2,6-diisopropylphenol) is a widely used intravenous anesthetic in clinic settings.Our previous study indicated that propofol inhibits mitochondrial reactive oxygen species (ROS) production via down-regulation of phosphatase A2 (PP2A) expression,inhibition of Ser36-p66shc dephosphorylation and mitochondrial translocation,thus improving high glucoseinduced endothelial injury.The expression of p66shc was inhibited by propofol in hyperglycemic human umbilical vein endothelial cells (HUVECs).However,the mechanism by which propofol inhibits p66shc expression in hyperglycemic HUVECs is still obscure.In the present study,we mainly examined how propofol inhibited high glucose-induced p66shc expression in HUVECs.Compared with 5mM glucose treatment,high glucose increased p66shc expression and decreased sift1 expression,which was inhibited by propofol treatment.Moreover,EX527 (a sirt1 inhibitor) reversed the effect of propofol against high glucose-induced p66shc expression.However,EX527 did not reverse the effects of propofol against high glucose-induced ROS accumulation,endothelial inflammation,and apoptosis.Furthermore,when cells were incubated with propofol,EX527,and FTY720 (a PP2A activator) simultaneously,the effects of propofol against high glucose-induced ROS accumulation,inflammation,and apoptosis were reversed.Our results suggested that propofol inhibited high glucose-induced p66shc expression via upregulation of sirt1 expression in hyperglycemic HUVECs.Moreover,propofol protects against high glucosemediated ROS accumulation and endothelial injury via both inhibition of p66shc expression and dephosphorylation of Ser36-p66shc.
其他文献
Chondrocyte is involved in the destruction of joints in osteoarthritis (OA) patients.The aim of this study was to explore the expression level of small nucleola
红叶桃春季新叶初发时叶色红艳,在夏季高温下会出现“返青”现象,且随着叶龄的增加,基部叶片叶色逐渐由暗红转绿,花色素苷含量下降。与之相反,许多早熟桃品种为夏秋季红叶型,
高效稳定的离体再生体系是开展遗传转化工作的前提。本试验以桃砧木毛桃(Prunus persica)的无菌试管苗为材料,在实验室已有的研究基础上,首先对叶片诱导出的愈伤组织进行石蜡
创造性思维是记者在新闻采写过程中,实现某些完全的创造和局部创新的科学有效的思维方式。正确认识和把握记者创造性思维的特征,对于记者在新闻工作实践中,开拓认识客观报道
In study,we aimed to determine the mechanisms underlying the gastroprotective effects of sodium copper chlorophyllin (SCC) against ethanol-induced gastric ulcer
为了探讨甘蔗与菜用大豆间种对其产量品质及土壤理化性状的影响,在甘蔗品种桂糖29中间种菜用大豆,进行以下几个方面研究:(1)甘蔗与菜用大豆间种对菜用大豆生长品质的影响;(2)甘蔗与菜用大豆间种对土壤理化性质的影响;(3)菜用大豆间种密度对菜用大豆光合特性的影响;(4)甘蔗与菜用大豆间种对甘蔗和菜用大豆产量性状的影响。研究结果如下:1间种提高了菜用大豆植株高度、叶面积指数、干物质积累量和甘蔗的锤度,但
Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer with poor clinical outcomes and without effective targeted therapies.Numero
Hypoxic-ischemic encephalopathy (HIE) is a complex condition which is associated with high mortality and morbidity.However,few promising treatments for HIE exis
运用个案分析,反思翻译变体的分类和操作问题,以期表明,虽然可以从理论上将翻译变体划分为不同类型,实践中却常常是各种策略类型交融难分的.
MicroRNA-200b (miR-200b) down-regulation has been found in wound-healing tissues.Fibroblasts are the predominant cells that orchestrate the production of collag