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目的探讨玉米紫色植株色素对氟中毒大鼠的抗氧化作用。方法离乳健康SD大鼠80只,雌雄各半,动物室适应性喂养1周后,按体重均衡原则随机分为4组,每组亦雌雄各半,分别为对照组、氟中毒组、低剂量色素组和高剂量色素组。对照组饮普通自来水,饲普通饲料,其他3组饮含F-100.00 mg/L的自来水;氟中毒组饲普通饲料,低剂量色素组和高剂量色素组分别饲含玉米紫色植株色素为5 g/kg和10 g/kg的普通饲料,实验周期为12周。实验结束后,检测各组大鼠血清和肝组织中抗氧化指标。结果与对照组比较,各染氟组大鼠血清、尿液和肝组织中的F-含量显著增高(P<0.01);氟中毒组大鼠血中MDA含量(4.27±1.65 nmol/ml)分别显著高于对照组(3.34±1.08nmol/ml)和低剂量色素组(3.19±0.81 nmol/ml)(P<0.05、P<0.01),氟中毒组大鼠血中GSH-Px含量(1 295.68±240.84酶活力单位)低于低剂量色素组(1 462.25±341.01酶活力单位)(P<0.05);氟中毒组大鼠肝脏MDA(2.60±1.53 nmol/mgprot)明显高于对照组(1.87±0.78 nmol/mgprot)(P<0.05),低剂量色素组和高剂量色素组大鼠肝脏GSH(4.15±1.39和4.61±2.11 mg/gprot)均高于氟中毒组(2.90±1.36 mg/gprot)(P<0.05,P<0.01)。结论玉米紫色植株色素可提高氟中毒大鼠机体组织抗氧化能力。
Objective To investigate the antioxidative effect of maize purple plant pigment on fluorosis rats. Methods Eighty healthy male Sprague-Dawley rats were randomly divided into 4 groups, each of which was male and female, according to the principle of weight balance. They were control group, fluorosis group, low Dose pigment group and high-dose pigment group. The control group drinking ordinary tap water, feeding ordinary feed, the other three groups drinking water containing F-100.00 mg / L; fluoride poisoning group fed normal feed, low-dose pigment group and high-dose pigment group containing corn purple plant pigment 5 g / kg and 10 g / kg of common feed, the experimental period of 12 weeks. After the experiment, anti-oxidation index in serum and liver of each group was detected. Results Compared with the control group, the levels of F- in the serum, urine and liver tissues of the rats with fluorosis were significantly increased (P <0.01). The levels of MDA in the blood of fluorosis rats were 4.27 ± 1.65 nmol / ml (P <0.05, P <0.01). The content of GSH-Px in the blood of the rats with fluorosis was significantly higher than that of the control group (3.34 ± 1.08nmol / ml) and the low dose pigment group (3.19 ± 0.81nmol / ± 240.84 enzyme units) was lower than that in the low-dose group (1 462.25 ± 341.01 enzyme units) (P <0.05); the liver MDA content of fluorosis group was significantly higher than that of the control group (1.87 ± 1.53 nmol / mgprot) 0.78 nmol / mgprot) (P <0.05). The liver GSH (4.15 ± 1.39 and 4.61 ± 2.11 mg / gprot) in low and high dose pigment groups were significantly higher than those in fluorosis group (2.90 ± 1.36 mg / gprot) (P <0.05, P <0.01). Conclusion Purple maize plant pigment can improve the antioxidant capacity of fluoride poisoning in rats.