目的:建立反相高效液相色谱法测定格列美脲片含量及含量均匀度。方法:采用Zorbax SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),流动相为0.025 mol·L~(-1)磷酸二氢钾溶液(稀氢氧化钾溶液调节至pH 5.2)-甲醇(25:75),流速1 mL·min~(-1),检测波长为228 nm。结果:进样量在0.05～1.0 μg时,与峰面积呈良好线性,r=0.999 9(n=7),平均回收率99.97％,日内和日间RSD分别小于0.7％及0.8％。结论:该法简便、准确、专属。 Objective: To establish an RP-HPLC method for the determination of glimepiride tablets content and content uniformity. Methods: Zorbax SB-C_ (18) column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was 0.025 mol·L -1 potassium dihydrogen phosphate solution (adjusted to pH 5.2 with dilute potassium hydroxide solution) - methanol (25:75) at a flow rate of 1 mL · min ~ (-1). The detection wavelength was 228 nm. Results: When the injection volume was in the range of 0.05-1.0 μg, the peak area was linear with r = 0.999 9 (n = 7). The average recovery was 99.97%. The intra-day and inter-day RSD were less than 0.7% and 0.8% respectively. Conclusion: This method is simple, accurate and exclusive.