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BEL-7402细胞是上海细胞生物学研究所从人体肝癌标本建立的恶性上皮细胞系,能在体外连续培养,可用于肝癌细胞代谢、膜抗原及膜受体等的研究。本文介绍培养传代的方法和实践体会,供参考。材料与试剂 1、7402细胞株上海肿瘤研究所提供 2、“Nissui” RPMI 1640培养液 3、小牛血清 4、“三抗”Hank’s液链霉素1g、青霉素G100万单位、卡那霉素1g,溶于Hanks液100ml。 5、消化液 (1)0.1％EDTA液:EDTA 0.5g、Na Cl 4.0g, KCl 0.1g、Na_2 HPO_4 0.5g、KH_2PO_A 0.1g溶于500ml三蒸水中,调节pH
BEL-7402 cells is a malignant epithelial cell line established by Shanghai Institute of Cell Biology from human hepatoma specimens and can be cultured continuously in vitro for the study of liver cancer cell metabolism, membrane antigens and membrane receptors. This article describes the method of training and practice of experience, for reference. Materials and reagents 1,7402 cells Shanghai Institute of Oncology 2, “Nissui ” RPMI 1640 medium 3, bovine serum 4, “three anti” Hank’s liquid streptomycin 1g, penicillin G million units, kanamycin Su 1g, dissolved in Hanks solution 100ml. 5, digestive juice (1) 0.1% EDTA solution: EDTA 0.5g, NaCl 4.0g, KCl 0.1g, Na2HPO4 0.5g, KH2PO_A 0.1g dissolved in 500ml three steam water, adjust the pH