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目的:利用TRAP分子标记技术研究短葶山麦冬遗传多样性。方法:以果糖、光合或甾体皂苷代谢途径7个关键酶基因和1个凝集素基因作为靶标序列设计固定引物13条,筛选随机引物19条,利用其中11对多态性较高引物组合对50份短葶山麦冬种质进行分析。结果:共扩增了335条带,其中多态性带323条,占96.42%。引物平均多态信息量0.930。遗传分化系数0.610。UPGMA聚类表明相似系数0.52~0.98。结论:短葶山麦冬遗传多样性水平较高,出现了一定程度的遗传分化。
Objective: To study the genetic diversity of Ophiopogon japonicus by TRAP molecular marker. Methods: Thirteen primers were designed by using seven key enzyme genes of fructose, photosynthesis and steroidal saponin metabolism and one lectin gene as target sequences. Thirteen primers were screened for randomness and 11 pairs of primers with high polymorphism 50 copies of Ophiopogon japonicus germplasm were analyzed. Results: A total of 335 bands were amplified, of which 323 bands were polymorphic, accounting for 96.42%. The average polymorphic information of primer 0.930. Genetic differentiation coefficient of 0.610. UPGMA clustering showed similar coefficients of 0.52 ~ 0.98. Conclusion: There is a high level of genetic diversity in Ophiopogon japonicus, with a certain degree of genetic differentiation.