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蚜虫获毒酶联免疫吸附法（ＡＡ－ＥＬＩＳＡ）是指用ＥＬＩＳＡ测定从染病植株上获毒蚜虫以反映品种抗ＢＹＤＶ的方法。经对带Ｙｄ２基因大麦品种８７７－１６与感病品种Ｍａｎｌｅｙ对比测定，按其方法接种３叶期第１叶片，传毒３ｄ后用无毒禾谷类蚜虫从第２叶片上取食获毒１ｄ，采集５头作ＥＬＩＳＡ测定。ＡＡ－ＥＬＩＳＡ法测定ＯＤ值，Ｍａｎｌｅｙ显著高于８７７－１６，用ＥＬＩＳＡ直接测定无显著差异。用ＡＡ－ＥＬＩＳＡ法具有不破坏植株，可对同一部位重复测定，且蚜虫体软，易研碎抽提病毒，测定抗感品种差异明显等优点，具有适用性。
Aphid Enzyme-Linked Immunosorbent Assay (AA-ELISA) refers to the method of measuring the resistance to BYDV by using aphids collected from infected plants by ELISA. The first leaf of 3-leaf stage was inoculated according to the method of 877-16 with the Yd2 barley variety Manley and the susceptible variety Manley. After the virus was infected for 3 days, the non-toxic cereal aphid was taken from the second leaf for 1 day, 5 were collected for ELISA assay. The OD of Manley was significantly higher than that of 877-16 by AA-ELISA, and no significant difference was detected by ELISA. With AA-ELISA method does not damage the plant, the same site can be repeated determination, and aphids are soft, easy to crush virus extraction, determination of anti-susceptible varieties obvious advantages, is applicability.