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目的 探讨丙型肝炎病毒和乙型肝炎病毒感染在非霍奇金淋巴瘤发病中的作用 .方法 采用 EL ISA和反转录 -聚合酶链反应的方法检测了 74例非霍奇金淋巴瘤患者外周血的抗 - HCV抗体和 HCVRNA;同时检测了 110名健康献血员中抗 - HCV抗体和 HCVRNA.用 EL ISA方法检测了 74例非霍奇金氏淋巴瘤患者和 110名献血员 .同时采用聚合酶链反应对于非霍奇金淋巴瘤患者外周血淋巴细胞中 Bcl- 2 / JH 基因重排情况进行了检测 .结果 在 74例非霍奇金氏淋巴瘤中的抗 - HCV抗体阳性的为 4例 ,阳性率为 5 .3% ,HCVRNA阳性者为 6例 ,阳性率为 8.1% ;110名健康献血员的抗 - HCV抗体的阳性率为 0 .9% ;HCVRNA阳性率为 1.8% .非霍奇金淋巴瘤中的抗 - HCV抗体阳性率较健康献血员显著增高 ,而 HCVRNA的阳性率也较健康献血员有增高 .经 χ2检验 P>0 .0 5 ,无显著性差异 .在 74例非霍奇金淋巴瘤中 HBs Ag阳性者有 3例 (4% ) ,而在献血员中为 2名 (1.7% ) .在 74例非霍奇金淋巴瘤中有 18例外周血检测出 Bcl- 2 / JH基因重排 ,且转位均发生在 Bcl- 2的主要断裂点区域 (MBR) .经χ2 testP<0 .0 1,在非霍奇金淋巴瘤中 Bcl- 2 / JH基因重排和 HCV感染有明显的相关性 .结论 HCV和 HBV的感染与非霍奇金氏淋巴瘤的发生可能不存在一定的相?
Objective To investigate the role of hepatitis C virus and hepatitis B virus infection in the pathogenesis of non-Hodgkin’s lymphoma. METHODS: A total of 74 patients with non-Hodgkin’s lymphoma were examined by EL ISA and reverse transcription-polymerase chain reaction. Anti-HCV antibodies and HCV RNA in peripheral blood; anti-HCV antibodies and HCV RNA in 110 healthy blood donors were tested at the same time. 74 patients with non-Hodgkin’s lymphoma and 110 blood donors were tested with the EL ISA method. The polymerase chain reaction (PCR) was used to detect Bcl-2/JH gene rearrangement in peripheral blood lymphocytes in patients with non-Hodgkin’s lymphoma. The results were positive for anti-HCV antibodies in 74 cases of non-Hodgkin’s lymphoma. In 4 cases, the positive rate was 5.3%, HCVRNA positive was 6 cases, and the positive rate was 8.1%; 110 healthy blood donors had anti-HCV antibody positive rate of 0.9%; HCV RNA positive rate was 1.8%. The positive rate of anti-HCV antibody in non-Hodgkin’s lymphoma was significantly higher than that of healthy blood donors, and the positive rate of HCVRNA was also higher than that of healthy blood donors. There was no significant difference in χ2 test P>0.05. Among the 74 non-Hodgkin lymphomas, 3 were positive for HBsAg (4%), and 2 (1.7%) among blood donors. 18 Bcl-2/JH gene rearrangements were detected in 18 of 74 non-Hodgkin’s lymphomas, and all of the translocations occurred in The Bcl-2 major breakpoint region (MBR) was highly correlated with HCV infection in non-Hodgkin’s lymphoma by χ2 testP<0.01. Conclusion HCV and HBV The infection may not be in phase with the occurrence of non-Hodgkin’s lymphoma.