目的　探讨DNA检测对急性淋巴细胞性白血病 (ALL)诊断和治疗的临床意义。方法　应用流式细胞术检测了 35例急性淋巴细胞性白血病患者骨髓细胞DNA含量 (DI)、倍体和细胞增殖活性 (PI)。结果　ALL异倍体检出率为 5 1 4 % ( 18 35 ) ,其中亚二倍体 8例 ,超二倍体 10例 ;ALL组骨髓细胞G0 G1%高于对照组 (P <0 0 5 ) ,而S %、PI值明显低于对照组 (P <0 0 5 )。结论　DNA含量检测和倍体分析对急性淋巴细胞性白血病的诊断、治疗是很有价值的临床生物学指标 ,有助于白血病的准确、客观诊断和判断预后 Objective To investigate the clinical significance of DNA testing in the diagnosis and treatment of acute lymphoblastic leukemia (ALL). Methods The DNA content (DI), ploidy and cell proliferative activity (PI) of bone marrow cells from 35 patients with acute lymphoblastic leukemia were detected by flow cytometry. Results The detection rate of ALL aneuploidy was 5 1 4% (18 35), including 8 cases of sub-diploid and 10 cases of hyper-diploid. The G0 G1% of ALL group was higher than that of control group (P 0 05) , While the S% and PI values ​​were significantly lower than those in the control group (P <0.05). Conclusion DNA content and ploidy analysis are valuable clinical biomarkers for the diagnosis and treatment of acute lymphoblastic leukemia and contribute to the accurate and objective diagnosis and prognosis of leukemia