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目的:探讨NINJ2基因旁侧的rs11833579位点多态性与房山汉族人群脑卒中的关系。方法:采取横断面病例-对照相关分析。分别采用多聚酶链式反应-限制性片段长度多态性(PCR-RFLP)分析及直接测序技术检测NINJ2的基因型。脑卒中患者790人(其中脑梗塞679人),为北京房山第一医院2003～2008年住院患者;正常对照者811例,为房山区农村查体无脑卒中家族史的无血缘关系汉族正常人群。结果:NINJ2基因rs11833579位点的GG基因型和G等位基因频率在脑梗塞组较对照组明显升高,P均<0.001;脑出血组与对照组比较,G等位基因频率差异有统计学意义(P=0.005)。基因型对血糖、总胆固醇和甘油三酯的影响不大(P>0.05)。结论:NINJ2基因rs11833579位点多态性与房山汉族人群中脑卒中易感强相关。
Objective: To investigate the relationship between rs11833579 polymorphism beside NINJ2 gene and stroke in Han nationality in Fangshan. Methods: A cross-sectional case-control analysis was performed. The genotypes of NINJ2 were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis and direct sequencing. 790 stroke patients (including 679 cerebral infarction), Beijing Fangshan First Hospital from 2003 to 2008 hospitalized patients; 811 cases of normal controls, Fangshan District Rural stroke without family history of stroke-unrelated Han normal population . Results: The frequency of GG genotype and G allele in rs11833579 locus of NINJ2 gene in cerebral infarction group was significantly higher than that in control group (P <0.001). There was statistical difference in G allele frequency between cerebral hemorrhage group and control group Meaning (P = 0.005). Genotypes had little effect on blood glucose, total cholesterol and triglycerides (P> 0.05). Conclusion: The rs11833579 polymorphism of NINJ2 gene is strongly associated with stroke susceptibility in Fangshan Han population.