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1971年作者发现慢性淋巴性白血病者血清在丙烯酰胺凝胶圆盘电泳上产生一种特异图象。后来作者和其他研究者提出癌瘤病人血清中有免疫抑制蛋白存在。为此,作者进一步应用血清丙烯酰胺圆盘电泳术探查恶性肿瘤病人血清是否有特异的电泳现象。取健康人和肿瘤病人的血清,迅速冰冻且贮存于—60℃直至用时。制备10%或15%丙烯酰胺凝胶电泳柱,用10微升血清电泳,电流恒定于5 mA/柱,在25℃电泳55分钟。电泳后再用280毫微米波
In 1971, the authors found that serum from patients with chronic lymphocytic leukemia produced a specific image on acrylamide gel disk electrophoresis. Later, the authors and other researchers proposed the presence of immunosuppressive proteins in the serum of cancer patients. To this end, the author further application of serum acrylamide disk electrophoresis in patients with malignant tumors to explore whether there is a specific electrophoresis. Serum from healthy and cancer patients is rapidly frozen and stored at -60 ° C until use. A 10% or 15% acrylamide gel electrophoresis column was prepared, electrophoresed with 10 μl of serum, the current was constant at 5 mA / column, and electrophoresed at 25 ° C for 55 minutes. After electrophoresis with 280 nm wave