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分析了外源一氧化氮 (nitricoxide ,NO)供体硝普钠 (sodiumnitroprusside ,SNP)对离体小麦 (TriticumaestivumL .)叶片过氧化氢 (H2 O2 )含量及其清除酶活力的调节作用。不同浓度的SNP (1mmol/L和 5mmol/L)处理 3 0min内 ,离体小麦叶片H2 O2 含量均有一个显著上升的过程 ,同时过氧化物酶 (POD)活力受到显著抑制 ,而过氧化氢酶 (CAT)活力则轻微下降 ;处理 3 0min到 2 4 0min时 ,POD活力的抑制状态基本维持不变 ,而CAT活力开始恢复上升 ,H2 O2 含量也相应地开始下降。粗酶液的体外实验也表明 ,SNP对POD和CAT的抑制类型不同 ,前者可能是不可逆抑制 ,后者则可能是可逆抑制。因此NO可通过对POD和CAT的不同抑制作用来调节小麦叶片内源H2 O2 含量
The regulatory effect of exogenous nitric oxide (NO) donor sodiumnitroprusside (SNP) on H2O2 content and scavenging enzyme activity in leaves of Triticum aestivum L. was studied. H2O2 content in detached wheat leaves increased significantly with different concentrations of SNP (1 mmol / L and 5 mmol / L) for 30 min, while the activity of peroxidase (POD) was significantly inhibited. However, hydrogen peroxide The activity of CAT decreased slightly. From the time of 30 min to 240 min, the inhibition of POD activity basically remained unchanged while the activity of CAT began to recover and the content of H2 O2 began to decrease correspondingly. In vitro experiments of crude enzyme solution also showed that the inhibition of SNP on POD and CAT is different, the former may be irreversible inhibition, the latter may be reversible inhibition. Therefore NO can regulate the endogenous H 2 O 2 content of wheat leaves through different inhibition of POD and CAT