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建立用反相高效液相色谱法同时测定白花檵木花中槲皮素和山奈酚含量的方法。采用Eclipse XDB-C18色谱柱(4.6mm×250mm,5μm);柱温:25℃;流动相为乙腈-0.4%磷酸水溶液(35:65,V/V);流速为1.0mL·min-1;检测波长为360nm。槲皮素在1.08×10-3—1.08×10-1μg·μL-1的范围内具有良好的线性关系(r=0.9999),平均回收率97.23%,RSD为1.26%;山奈酚在1.04×10-3—1.04×10-1μg.μL-1的范围内具有良好的线性关系(r=0.9999),平均回收率99.78%,RSD为0.46%。方法简便、准确、可靠,可作为白花檵木花中槲皮素和山奈酚的定量分析方法。
To establish a method for the simultaneous determination of quercetin and kaempferol in white flowers of Alnus cremastogyne by reversed-phase high performance liquid chromatography. The column temperature was 25 ℃. The mobile phase was acetonitrile-0.4% phosphoric acid solution (35:65, V / V). The flow rate was 1.0 mL · min-1. The detection wavelength is 360nm. Quercetin had a good linearity (r = 0.9999) in the range of 1.08 × 10-3-1.08 × 10-1 μg · μL-1 with an average recovery of 97.23% and a RSD of 1.26%. In the range of 1.04 × 10 -3-1.04 × 10-1μg.μL-1, the average recovery was 99.78% and the RSD was 0.46%. The method is simple, accurate and reliable and can be used as a quantitative method for the quantitative analysis of quercetin and kaempferol in Alnus japonica.