Objective:To determine antibiotic resistance patterns and virulence potential of Campylobacter jejuni(C.jejuni) isolates from clinical human diarrheal infections,cattle and healthy broilers.Methods:Antibiotic sensitivity patterns of C.jejuni isolates were determined by Kirby Bauer Disc Diffusion assay.These isolates were then subjected to virulence profiling for the detection of map A(membrane-associated protein).cadF(fibronectin binding protein).wlaN(beta-1.3-galaclosyltransferase) and neu AB(sialic acid biosynthesis gene).Further C.jejuni isolates were grouped by random amplification of polymorphic DNA(RAPD) profiling.Results:A total of436 samples from poultry(n=88).cattle(n=216) and humans(n=132) from different locations were collected.Results revealed percentage of C.jejuni isolates were 35.2%(31/88).25.0%(54/216) and 11.3%(15/132) among poultry,cattle and clinical human samples respectively.Antibiotic susceptibility results showed that similar resistance patterns to cephalothin was ie.87.0%,87.1%and 89%among humans,poultry and cattle respectively,followed by sulfamethoxazolc+trimcthoprim 40.0%,38.7%and 31.0%in humans,poultry and cattle and Ampicillin 40%,32%and 20%in humans,poultry and cattle respectively.Beta-lactamase activity was detected in 40.00%humans.20.37%cattle and 32.25%in poultry C.jejuni isolates.CadF and mapA were present in all poultry,cattle and human C jejuni isolates.wlauN was not detected in any isolate and neu AB was found in 9/31(36%) poultry isolates.RAPD profiling results suggested high diversity of C.jejuni isolates.Conclusions:Detection of multidrug resistant C.jejuni strains from poultry and cattle is alarming as they can be potential hazard to humans.Moreover,predominant association of virulence factors,cadF and map A(100%each) in C.jejuni isolates from all sources and neuAB(36%) with poultry isolates suggest the potential source of transmission of diverse types of C.jejuni to humans. Objective: To determine antibiotic resistance patterns and virulence potential of Campylobacter jejuni (C. jejuni) isolates from clinical human diarrheal infections, cattle and healthy broilers. Methods: Antibiotic sensitivity patterns of C. jejuni isolates were determined by Kirby Bauer Disc Diffusion assay. isolates were then subjected to virulence profiling for the detection of map A (membrane-associated protein). cadF (fibronectin binding protein). wlaN (beta-1.3-galaclosyltransferase) and neu AB (sialic acid biosynthesis gene) were grouped by random amplification of polymorphic DNA (RAPD) profiling. Results: A total of 436 samples from poultry (n = 88) .cattle (n = 216) and humans C.jejuni isolates were 35.2% (31/88) .25.0% (54/216) and 11.3% (15/132) among poultry, cattle and clinical human samples respectively. Antibiotic susceptibility results showed similar similar resistance patterns to cephalothin wa 40.0%, 87.1% and 89.0% in humans, poultry and cattle respectively, followed by sulfamethoxazolc + trimcthoprim 40.0%, 38.7% and 31.0% in humans, poultry and cattle and Ampicillin 40%, 32% and 20% , poultry and cattle respectively.Beta-lactamase activity was detected in 40.00% humans.20.37% cattle and 32.25% in poultry C.jejuni isolates.CadF and mapA were present in all poultry, cattle and human C jejuni isolates.wlauN was not detected in any isolate and neu AB was found in 9/31 (36%) poultry isolates. RAPD profiling results suggested high diversity of C. jejuni isolates. Conclusion: Detection of multidrug resistant C. jejuni strains from poultry and cattle is alarming as they can be potential hazard to humans. Moreover, predominant association of virulence factors, cadF and map A (100% each) in C. jejuni isolates from all sources and neuAB (36%) with poultry isolates suggest the potential source of transmission of diverse types of C.jejuni to humans.