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据文献报导,秋水仙碱(Colchicine)在离体实验中能抑制胶原前体向细胞外转运,并刺激胶原酶的产生。因此,作者用四氯化碳造成大鼠肝硬化模型,观察放射性脯氨酸掺入胶原脯氨酸(Collagen-proline)和胶原羟基脯氨酸的量。结果发现,每日口服秋水仙碱10微克的6只鼠,其掺入量比对照组10只鼠低得多: 在肝硬化大鼠中~(14)C-脯氨酸掺入肝蛋白的量(d.p.m./克) 胶原脯氨酸羟脯氨酸非胶原脯氨酸对照组 18045±5232 1022±253 71158±8576 给药组 9960±2140 639±131 75345±2902 在提取肝脏胶原后观察脯氨酸渗入残余蛋白的量,没有观察到显著变化。而且经秋水仙碱治疗的动物肝功能试验明显好转,胆红质从1.9±0.4降到0.4±0.1毫克/100毫升,血清白蛋白从1.9±0.6上升到3.2±0.8克/100毫升。因为实验中没有发现秋水仙碱有任何严重的毒性反应,故在临床选择7例
It has been reported in the literature that colchicine can inhibit the migration of collagen precursors to the outside of cells and stimulate collagenase production in vitro. Therefore, the authors used carbon tetrachloride to induce rat cirrhosis models and observed the amount of radioactive proline incorporated into collagen-proline and collagen hydroxyproline. As a result, it was found that 6 mice, in which 10 μg of colchicine per day was orally administered, were incorporated in a much lower amount than 10 mice in the control group. In cirrhotic rats, the incorporation of 14C-proline into heparin Quantity (dpm / g) Collagen Proline Hydroxyproline Non-collagen Proline Control 18045 ± 5232 1022 ± 253 71158 ± 8576 Administered group 9960 ± 2140 639 ± 131 75345 ± 2902 Observed after prolonged extraction of liver collagen No significant change was observed in the amount of acid infiltrated the residual protein. And colchicine treatment of animals significantly improved liver function tests, bilirubin from 1.9 ± 0.4 to 0.4 ± 0.1 mg / 100 ml, serum albumin increased from 1.9 ± 0.6 3.2 ± 0.8 g / 100 ml. Because the experiment did not find any serious colchicine toxicity, it is the clinical choice of 7 cases