一、粪便滤液的制法:用容量为0.8—1.0毫升的特制的玻璃小匙,取粪便1匙放於盛有5毫升生理盐水的乳钵中,研磨3—5分钟使之均匀後以滤纸过滤,得透明液(偶遇以Seitz器亦滤不透明者),此液即为诊断用的粪便滤液。二、诊断液的制法:取24小时的培养茵(志贺、Flexner、Hiss三型菌)用生理盐水稀释至1毫升含40忆菌体(如用活菌作诊断液时每毫升含10亿菌体),加甲醛,在室温内放置24小时,然 First, the faeces filtrate of the law: with a capacity of 0.8-1.0 ml of specially made glass teaspoon, take 1 drop of feces into a mortar containing 5 ml of normal saline, grinding 3-5 minutes so that after the filter paper Filtration, in the transparent liquid (occasionally encountered Seitz also filter opacity), this solution is diagnostic fecal filtrate. Second, the diagnostic system of law: take 24-hour incubation Yin (Shiga, Flexner, Hiss Type III) diluted to 1 ml with normal saline containing 40 strains of bacteria (such as with live bacteria as diagnostic fluid containing 10 Billion bacteria), plus formaldehyde, at room temperature for 24 hours, of course