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目的以急性铜绿假单胞菌肺部感染的小鼠为研究对象,探讨白细胞介素17(interleukin-17,IL-17)在铜绿假单胞菌肺部感染中的变化及可能机制。方法经气道内注入铜绿假单胞菌建立急性肺部感染小鼠模型,随后收集3d的动物肺组织、支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)及外周血,采用实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RQ-PCR)和酶联免疫吸附剂测定(enzyme-linked immunosorbent assay,ELISA)方法检测IL-17以及炎性因子IL-1β、粒细胞集落刺激因子(granulocyte colony stimulating factor,G-CSF)的表达水平。结果急性铜绿假单胞菌肺部感染后的小鼠肺组织IL-17的mRNA水平显著高于对照组,肺泡灌洗液中IL-17的蛋白水平也显著高于对照组,两组比较差异具有统计学意义(P<0.05)。感染组肺泡灌洗液中IL-1β及G-CSF的表达水平在3 d内显著高于对照组,两组比较差异具有统计学意义(P<0.05)。结论 IL-17可能通过其下游因子IL-1β和G-CSF的作用使气道中性粒细胞增加,从而在早期发挥对急性铜绿假单胞菌肺部感染的保护作用。
Objective To investigate the changes and possible mechanisms of interleukin-17 (IL-17) in the pulmonary infection of Pseudomonas aeruginosa in mice infected with Pseudomonas aeruginosa lung. Methods Acute lung infection in mice was established by injecting Pseudomonas aeruginosa into the airway and then collecting the animal lung tissue, bronchoalveolar lavage fluid (BALF) and peripheral blood of 3 days. Real-time fluorescent quantitative polymerase chain reaction (RQ-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect the expressions of IL-17, IL-1β and granulocyte colony stimulating factor, G-CSF). Results The mRNA level of IL-17 in the lungs of mice infected with Pseudomonas aeruginosa was significantly higher than that in the control group, and the protein level of IL-17 in BALF was also significantly higher than that in the control group Statistically significant (P <0.05). The levels of IL-1β and G-CSF in BALF in infected group were significantly higher than those in control group within 3 days, with significant difference between the two groups (P <0.05). Conclusion IL-17 may increase airway neutrophils through the action of its downstream factors IL-1β and G-CSF, which may exert its protective effect on acute pulmonary infection of Pseudomonas aeruginosa in the early stage.