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2000-2006年,从广西8个番茄产区采集了508份番茄晚疫病标样,分离纯化获得239个晚疫病菌株。交配型鉴定结果表明,广西番茄晚疫病菌以A1交配型为主,占总菌株数的96.65%;发现8个A2交配型菌株,分布于田阳县、田东县、武鸣县和柳州市。利用6个含有不同单显性抗番茄晚疫病基因的番茄材料T s19、T s33、W.V a700、CLN 2037B、L 3708和LA 1033为鉴别寄主,初步鉴定了来自7个番茄产区32个代表菌株的生理小种,结果共鉴定出5个小种,即T0、T1、T1,2、T1,2,4、T1,2,3,4,5;其中小种T1,2和T1,2,3,4,5是优势小种,分别广泛分布于5个和3个番茄产区,出现频率分别占37.50%、34.37%;其次是小种T0和T1,分别分布于5个和3个番茄产区,出现频率分别占15.63%和9.37%;小种T1,2,4仅在田阳县发现,出现频率为3.13%。这表明广西番茄晚疫病病菌具有复杂的多样性。
From 2000 to 2006, 508 tomato late blight standard samples were collected from 8 tomato producing areas in Guangxi, and 239 late blight isolates were isolated and purified. The result of mating type identification showed that the dominant tomato mating type was Phytophthora A1 in Guangxi, accounting for 96.65% of the total number of strains. Eight A2 mating strains were found in Tianyang, Tiandong, Wuming and Liuzhou. Six tomato cultivars T s19, T s33, WV a700, CLN 2037B, L 3708 and LA 1033 with different dominant single resistance to late blight of tomato were used as differential hosts to identify 32 representative strains from 7 tomato producing areas The results showed that 5 minor species were identified as T0, T1, T1, 2, T1, 2, 4, T1, 2, 3, 3,4,5 were the dominant races, which were widely distributed in 5 and 3 tomato producing areas respectively, the frequency of occurrence was 37.50% and 34.37% respectively; the next was T0 and T1, which were distributed in 5 and 3 tomato The frequency of occurrence in the producing areas accounted for 15.63% and 9.37% respectively; the races T1, 2 and 4 were only found in Tianyang County, with a frequency of 3.13%. This shows that the tomato tomato blight in Guangxi has a complex diversity.