建立家兔血浆中新抗炎镇痛剂 SFZ- 47[(± ) - 3H - 1 ,2 -二氢 - 2 - ( 4 -甲基苯胺基 ) -甲基 - 1 -吡咯里嗪酮 ]对映异构体浓度测定的高效液相色谱 ( HPL C)测定法 .采用反相 HPLC测定服药后血浆中 (± ) - SFZ- 47,同时收集其流出组分 .经浓缩后 ,再用 Chiral- AGP手性色谱柱进行对映异构体的直接分离 ,达到了良好的分离效果 .实验表明 ,动物体内药物动力学过程具有明显的立体选择性 To establish a new anti-inflammatory and analgesic agent SFZ-47 [(±) - 3H - 1, 2 - dihydro - 2 - (4 - methylanilino) - methyl - 1 - pyrrolizinone] Determination of enantiomer concentration by high performance liquid chromatography (HPL C) Determination of (±) - SFZ-47 in plasma by reverse phase HPLC, while collecting its effluent components.After concentration, and then Chiral- AGP chiral column for the direct separation of enantiomers, to achieve a good separation effect. Experiments show that the pharmacokinetics of animals in vivo process has obvious stereoselectivity