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为探究胎盘中胰岛素样生长因子1的浓度和基因甲基化变化与宫内生长受阻(IUGR)发生的关系。选择56名正常妊娠足月分娩的产妇和新生儿为对象,其中27名IUGR患儿,29名正常出生体重儿,收集产妇及新生儿的基本信息和胎盘样本。用实时荧光定量PCR检测IGF-1 m RNA表达量,用BSP分析胎盘IGF-1基因启动子区Cp G位点的甲基化水平。IUGR组IGF-1 m RNA的ΔCT值为(7.483±1.406),对照组为(5.642±1.323),经t检验,t=3.567,p=0.001(p<0.01),提示IUGR患者胎盘IGF m RNA表达低于对照组。胎盘IGF-1启动子区Cp G位点均呈高甲基化状态,两组间平均甲基化率的差异无统计学意义,但两组中男性和女性IGF-1甲基化率存在差异。IGF-1在IUGR的发生中起着重要的作用,一定水平的IGF-1对维持胎儿生长是至关重要的,其表达量的高低均与处于高甲基化状态的胎盘IGF-1的甲基化程度无关,与性别有关。
To explore the relationship between placental insulin-like growth factor-1 concentration and gene methylation change and intrauterine growth restriction (IUGR). Fifty-six mothers and newborns with full-term delivery of normal pregnancy were selected. Twenty-seven children with IUGR and 29 normal birth weight children were recruited. Basic information on maternal and newborn babies and placenta samples were collected. The expression of IGF-1 mRNA was detected by real-time fluorescence quantitative PCR, and the methylation level of CpG site in the promoter region of placental IGF-1 gene was analyzed by BSP. The ΔCT value of IGF-1 mRNA in IUGR group was (7.483 ± 1.406) and that of control group (5.642 ± 1.323), t = 3.567, p = 0.001 by t test (p <0.01) The expression was lower than the control group. CpG sites in the promoter region of placenta IGF-1 were hypermethylated with no significant difference in mean methylation rates between the two groups, but there was a difference in the methylation rates of IGF-1 between males and females in both groups. IGF-1 plays an important role in the development of IUGR. A certain level of IGF-1 is crucial for the maintenance of fetal growth. The level of IGF-1 is related to the methylation of placental IGF-1 in hypermethylated state The degree has nothing to do with gender.