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目的:对抗癌散的抗肿瘤活性成分进行研究。方法:用硅胶、葡聚糖凝胶(Sephadex LH-20)柱色谱等方法进行分离纯化,通过理化性质及光谱数据确定化合物的结构;采用流式细胞术(FCM)对部分单体化合物进行抗肿瘤活性的测定。结果:分离得到β-谷甾醇(Ⅰ)、正丁基-β-D-吡喃果糖苷(Ⅱ)、人参皂苷Rg_1(Ⅲ)3个化合物;活性实验结果表明,部分化合物具有一定的抗肿瘤活性。结论:本研究初步阐明了复方抗癌散中的主要化学成分,其中化合物Ⅱ为首次从该植物中分离得到;化合物(Ⅱ)和化合物(Ⅲ)对人肝癌细胞(Bel-7402)有较好的抑制活性。
Objective: To study the antitumor activity of Kangai San. METHODS: The compounds were isolated and purified by silica gel and Sephadex LH-20 column chromatography. The structures of the compounds were determined by physicochemical properties and spectral data. Some of the compounds were isolated by flow cytometry (FCM) Determination of tumor activity. Results: Three compounds, β-sitosterol (Ⅰ), n-butyl-β-D-fructopyranoside (Ⅱ) and ginsenoside Rg_1 (Ⅲ) were isolated and the results of their activity tests showed that some compounds have some anti-tumor active. Conclusion: The main chemical components of compound FangCaoCao San were elucidated in this study. Compound Ⅱ was isolated from this plant for the first time. Compound (Ⅱ) and compound (Ⅲ) showed good activity on Bel-7402 Inhibitory activity.