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以水稻成熟种胚为外植体,研究150Gy~(137)Csγ射线对籼、粳和糯稻三大类型各7个品种体细胞组织培养的效应。结果表明,γ射线对不同类型品种的离体培养效应各不相同。其水稻成熟种胚出愈率、绿苗分化率受抑制程度总的趋势是籼稻>粳稻>糯稻。初代培养时,愈伤组织生长速度受抑制程度与之相反。继代培养的正常型愈率和生长速度受抑制程度较为复杂。而且不同品种对辐射的敏感性在各个培养阶段表现出不同的效应。少数品种在某一培养阶段也有促进的现象。
The mature embryo of rice was used as explants to study the effect of 150Gy ~ (137) Csγ-rays on somatic tissue culture of seven varieties of indica, japonica and glutinous rice. The results showed that γ-rays had different effects on the in vitro culture of different types of cultivars. The rice ripening embryos out of the rate of green plantlet differentiation rate inhibited the general trend of indica> japonica> glutinous rice. The primary culture, callus growth rate inhibition by the opposite. Subculture of normal rate and growth rate of the inhibition of more complex. And the sensitivity of different species to radiation shows different effects in different stages of culture. A small number of species in a culture stage also promote the phenomenon.