在pH2.7的BR缓冲介质中,变色酸2R与蛋白质能形成复合物,最大吸收峰的波长为567nm,比试剂本身红移约65nm。实验制定的几种蛋白质的标准工作曲线,线性范围很宽,灵敏度高。用于实际人血清样品测定,准确性确度高,无干扰。本方法试剂及反应体系稳定、测定手续简便、重现性好。 In pH 2.7 BR buffering medium, chromotropic acid 2R forms a complex with the protein with a wavelength of maximum absorption peak of 567 nm, which is red-shifted by about 65 nm from the reagent itself. Experiment developed several standard protein working curve, a wide linear range, high sensitivity. For the actual human serum sample determination, the accuracy of a high degree of accuracy, non-interference. The method reagent and the reaction system are stable, the determination procedure is simple and the reproducibility is good.