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目的研究肿瘤细胞表面HLA分子表达及IFN-γ的调控作用。检测TIL细胞中T细胞亚群的变化,并与PBMC比较。方法采用FACS检测细胞表面的CD抗原,采用ABC免疫组化染色检测肿瘤细胞HLA-I、II类分子的表达。以IFN-γ诱导建系的卵巢癌细胞表达HLA分子。结果肾细胞癌患者TIL细胞中的T细胞明显多于大肠癌患者。肿瘤患者PBMC中的T细胞数少于正常对照;肿瘤组织HLA-I类抗原的表达明显低于正常组织,HLA-II类抗原的表达高于正常组织。与正常组织相比较,大肠癌患者比肾细胞癌患者HLA-I类抗原的表达更低。IFN-γ可诱导建系卵巢癌细胞表达HLA-I类抗原,但不能诱导HLA-II类抗原的表达。结论肿瘤组织HLA表达的变化与肿瘤组织中TIL的数有关;IFN-γ能选择性地诱导卵巢癌细胞表达HLA-I类分子,在抗肿瘤免疫中具有重要的意义。
Objective To study the expression of HLA molecules on the surface of tumor cells and the regulation of IFN-γ. Changes in T cell subsets were detected in TIL cells and compared with PBMCs. Methods The cell surface CD antigen was detected by FACS and the expression of HLA-I and II molecules was detected by ABC immunohistochemical staining. HLA molecules were expressed in ovary cancer cells induced by IFN-γ. Results The number of T cells in TIL cells of renal cell carcinoma patients was significantly higher than that of colorectal cancer patients. The number of T cells in PBMCs of tumor patients was less than normal controls; the expression of HLA-I antigens in tumor tissues was significantly lower than that in normal tissues, and the expression of HLA-II antigens was higher than that in normal tissues. Compared with normal tissues, patients with colorectal cancer have lower expression of HLA-I antigens than those with renal cell carcinoma. IFN-γ can induce ovarian cancer cells to express HLA-I antigens but not HLA-II antigens. Conclusion The change of HLA expression in tumor tissue is related to the number of TIL in tumor tissue. IFN-γ can selectively induce ovarian cancer cells to express HLA-I molecules, which has important significance in anti-tumor immunity.