论文部分内容阅读
利用RAPD技术对芥菜(BrasicajunceaCos.)16个变种的遗传变异进行研究。从60个随机引物中筛选出27个有效引物,共扩增出336条DNA带,其中275条为多态性带,占8185%,平均每个引物扩增的DNA带数为1244条。利用19个有效引物扩增的240条DNA带对芥菜16个变种间的亲缘关系进行UPGA聚类分析,计算出16个变种间的平均遗传距离为734,在此基础上建立了中国菜用芥菜16个变种的DNA分子系统树图。该系统将芥菜的16个变种划归A、B、C三个组,其中A组7个变种,B组8个变种,C组只有1个变种,B组又可细分为两个亚组。对芥菜遗传多样性的分子基础进行了探讨。
The genetic variations of 16 varieties of Brassica juncea (Coss.) Were studied by RAPD technique. 27 effective primers were screened out from 60 random primers, 336 DNA bands were amplified, of which 275 were polymorphic, accounting for 8185%, with an average of 12 DNA bands per primer. 44 articles. Using 240 DNA bands amplified by 19 effective primers, we analyzed the genetic relationship among 16 varieties of mustard by UPGA cluster analysis, and calculated the average genetic distance between the 16 varieties as 734, on this basis, established Chinese food Tree molecular map of 16 varieties of mustard. The system will be 16 varieties of mustard under the A, B, C three groups, of which A group of seven varieties, B group of eight varieties, C group only one variant, B group can be subdivided into two subgroups . The molecular basis of genetic diversity in B. juncea was discussed.