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目的:研究萜烯类经皮促透剂桉树醇(1,8-Cineole)对HaCaT细胞膜流动性及细胞内Ca2+浓度的影响,探究其促透作用机制。方法:采用CCK-8试剂盒测定并计算桉树醇对HaCaT细胞的半数抑制率(IC50);利用荧光漂白恢复技术(FRAP)测定不同浓度桉树醇对HaCaT细胞膜流动性的影响;采用流式细胞仪测定桉树醇对HaCaT细胞内Ca2+浓度的改变。结果:桉树醇药物IC50为9.077 mmol·L-1;不同浓度桉树醇均可显著增加细胞膜流动性,且随着药物浓度的增大而增加,呈剂量依赖性关系。同时,桉树醇可降低HaCaT细胞内Ca2+浓度。结论:桉树醇的经皮促透作用机制可能与影响角质细胞内Ca2+浓度进而改变细胞膜流动性有关。
Objective: To study the effect of 1,8-Cineole, a transdermal percutaneous transdermal agent of terpene, on the fluidity and intracellular Ca2 + concentration of HaCaT cell membrane, and to investigate the mechanism of its permeability enhancement. Methods: The half inhibitory rate (IC50) of eucalyptol on HaCaT cells was determined and calculated by CCK-8 kit. Fluorescence bleaching recovery (FRAP) was used to determine the effect of eucalyptol on the membrane fluidity of HaCaT cells. Flow cytometry Determination of eucalyptol on HaCaT cells Ca2 + concentration changes. Results: Eucalyptol drug IC50 was 9.077 mmol·L -1. Eucalyptol at different concentrations significantly increased cell membrane fluidity and increased with the increase of drug concentration in a dose-dependent manner. At the same time, eucalyptol can reduce the intracellular Ca2 + concentration in HaCaT cells. CONCLUSION: The mechanism of percutaneous penetration of eucalyptol may be related to the effect of Ca2 + concentration in keratinocytes and the change of membrane fluidity.