目的:通过模拟溶血程度(即血浆中血红蛋白浓度)探索溶血程度对凝血酶原时间(PT)和部分活化凝血酶原时间(APTT)是否存在干扰,干扰程度是否在临床可接受的范围内.方法:选取2019年7月至2020年6月入院的203例患儿作为研究对象,检测三种模拟溶血水平下的PT、APTT,通过计算干扰值和相对偏倚,判断溶血程度对PT、APTT的干扰程度及对PT、APTT的影响是否可接受.结果:随着模拟溶血程度的增加,Hb对PT的干扰不明显,对APTT的干扰明显,呈负相关.溶血程度增加时,APTT相对偏倚> 10％,Hb对“,”Objective: To explore whether the degree of hemolysis interferes with prothrombin time (PT) and partially activated prothrombin time (APTT) by simulating the degree of hemolysis, and whether the degree of interference is within the clinically acceptable range. Methods A total of 203 children who were admitted to the hospital from July 2019 to June 2020 were selected as the research subjects to detect PT and APTT under three simulated hemolysis levels. By calculating the interference value and relative bias, the interference degree of the degree of hemolysis on PT and APTT was determined. And whether the impact on PT and APTT is acceptable. Results With the increase of the simulated hemolysis degree, the interference of Hb on PT was not obvious, but the interference on APTT was obvious, which was negatively correlated. When the degree of hemolysis increased, the relative bias of APTT was more than 10％, and the influence of Hb was unacceptable. Conclusion The effect of hemolysis on PT is acceptable, but it has a greater effect on APTT.