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目的 :了解 CD95在 HIV感染者记忆型和处女型 CD4 + T细胞表面的表达情况 ,探讨 Fas/ CD95在 HIV感染中的作用。方法 :采用流式细胞检测仪 (FACS)对 HIV感染者外周血 CD4 + T细胞表面 CD95、CD4 5 RA、CD4 5 RO的表达进行分析 ,用 EL ISA检测血清中 Fas水平。结果 :HIV感染后 ,血清 Fas水平随疾病的进展而逐渐升高 ,同时伴有处女型 T细胞表面 CD95表达增高以及记忆型 T细胞 CD95表达逐渐下降。结论 :Fas参与了 HIV的感染过程。研究 Fas和 CD95、CD4 5 RA、CD4 5 RO之间的关系 ,可以加深对 HIV致病机制的认识 ,并可了解疾病的进展情况和指导治疗。
Objective: To investigate the expression of CD95 on memory and virgin CD4 + T cells in HIV-infected patients and to explore the role of Fas / CD95 in HIV infection. Methods: The expression of CD95, CD4 5 RA and CD4 5 RO on CD4 + T cells in peripheral blood of HIV infected persons were analyzed by flow cytometry (FACS). The level of Fas in serum was detected by ELISA. Results: After HIV infection, the level of Fas in serum increased gradually with the progression of the disease, accompanied by an increase of CD95 expression on virgin T cells and a decrease of CD95 expression in memory T cells. Conclusion: Fas participates in the process of HIV infection. Studying the relationship between Fas and CD95, CD4 5 RA and CD4 5 RO can deepen the understanding of the pathogenesis of HIV and understand the progress of the disease and guide the treatment.