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本文提出了一种新型的抗体固定方法,并利用该方法制备了免疫场效应型传感器用于糖化血红蛋白水平的检测.首先,纳米金颗粒由混合自组装膜(SAMs)包裹形成一种功能化的纳米团簇.然后这种纳米团簇以自组装的方式固定在传感器金电极表面.之后,葡萄糖球菌蛋白A(SPA)被固定在纳米团簇表面用来定向固定抗体.利用该方法,纳米团簇可在金电极表面均匀分布.由此形成的多层生物膜可定向固定抗体,与抗体具有稳定的结合力,能较好地屏蔽噪声干扰,且与场效应型传感器兼容.利用该传感器对糖化血红蛋白和血红蛋白浓度检测,灵敏度分别为152.8μV.ng-1.mL和13.5μV.ng-1.mL.实验结果表明,该传感器具有较好的一致性和准确度,未来有望发展成为一种微型的用于糖尿病监控的糖化血红蛋白传感器.
In this paper, we propose a new method of antibody immobilization and use this method to prepare immunosensor-based sensors for the detection of glycated hemoglobin.Firstly, nano-gold particles are encapsulated by mixed SAMs to form a functionalized This cluster of nanoclusters is then immobilized on the surface of the sensor gold electrode in a self-assembled manner, and then the glucanococcal protein A (SPA) is immobilized on the surface of the nanocluster to orientate the immobilized antibody. With this method, Clusters can be evenly distributed on the surface of the gold electrode, and the multilayer biofilm thus formed can orientally fix the antibody, has stable binding force with the antibody, can shield the noise interference well and is compatible with the field effect sensor, The sensitivity of HbA1c and HbA1c were 152.8μV.ng-1.mL and 13.5μV.ng-1.mL respectively.The experimental results show that the sensor has better consistency and accuracy and is expected to develop into a kind of Miniature glycated hemoglobin sensor for diabetes monitoring.