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目的:观察Pokemon基因敲减后Lovo细胞对药物(dehydrocostus lactone,DHC)增殖抑制敏感性的变化。方法:构建Pokemon敲减的Lovo细胞稳定细胞株Lovo-KD,用荧光定量PCR检测Pokemon基因敲减效率,分别在Lovo-NC、Lovo-KD细胞中加入各浓度梯度的去氢木香内酯(dehydrocostus lactone,DHC),分别在24h、48h、72h用MTT法检测细胞增殖抑制效率。结果:与Lovo-NC组细胞相比,相同梯度DHC(10、15、20μg/ml)对Lovo-KD细胞24h、48h、72h的增殖抑制率均显著升高,如20μg/ml DHC作用48 h后Lovo-KD、Lovo-NC组细胞增殖抑制率分别为82.11±2.3%、28.93±2.4%,P<0.01。结论:Pokemon基因敲减可增加Lovo细胞对DHC药物增殖抑制敏感性。
Objective: To observe the change of sensitivity of Lovo cells to the inhibition of proliferation of dehydrocostus lactone (DHC) after knockdown of Pokemon gene. METHODS: Povomon-knockdown Lovo cell-stable cell line Lovo-KD was constructed. The efficiency of knockdown of Pokemon gene was detected by real-time fluorescence quantitative PCR. Respectively, deproteinization of Lovo-KD cells dehydrocostus lactone (DHC). The inhibitory rate of cell proliferation was detected by MTT assay at 24h, 48h and 72h respectively. Results: Compared with Lovo-NC group, the proliferation inhibition rates of Lovo-KD cells at 24h, 48h and 72h were significantly increased by the same gradient of DHC (10,15,20μg / ml) The cell proliferation inhibition rates in Lovo-KD and Lovo-NC groups were 82.11 ± 2.3% and 28.93 ± 2.4%, respectively, P <0.01. Conclusion: Pokemon knockdown can increase the sensitivity of Lovo cells to the inhibition of DHC proliferation.