论文部分内容阅读
目的:探讨氟伐他汀(fluvastatin,Flu)对高糖腹透液(high-glucose peritoneal dialysate,HGPDS)诱导人腹膜间皮细胞(human peritoneal mesothelial cells,HPMCs)纤维连接蛋白(fibronectin,FN)表达的影响。方法:体外培养HPMCs,同步化24 h后,分为正常对照组、HGPDS组、HGPDS+Flu组、单纯氟伐他汀组、DMSO对照组,各组分别刺激不同时间后,噻唑蓝(MTT)检测各组细胞的活力,RT-PCR检测FN的mRNA表达,ELISA法检测上清液FN蛋白表达的变化。结果:与正常对照组比较,HGPDS明显抑制细胞活力,HGPDS联合Flu共同培养24、36 h,细胞活力有所恢复,其中,1×10-6mol/L Flu作用24 h,细胞活力改善差异有统计学意义(P<0.05);与正常对照组比较,HGPDS明显增加人腹膜间皮细胞FN mRNA及蛋白表达(P<0.05),并呈时间依赖性,其中FN mRNA于6 h达高峰,FN蛋白于24 h达高峰。Flu可抑制HGPDS诱导的FN的高表达(P<0.05),并呈浓度依赖性。结论:HGPDS诱导体外培养人腹膜间皮细胞FN表达增加,此作用可被Flu抑制。
Objective: To investigate the effect of fluvastatin on the expression of fibronectin (FN) in human peritoneal mesothelial cells (HPMCs) induced by high-glucose peritoneal dialysate (HGPDS) influences. Methods: HPMCs were cultured in vitro for 24 h and divided into normal control group, HGPDS group, HGPDS + Flu group, fluvastatin group alone and DMSO control group. Thiazolyl tetrazolium (MTT) assay The viability of each group of cells was measured by RT-PCR FN mRNA expression, ELISA assay FN protein expression in the supernatant. Results: Compared with the normal control group, HGPDS significantly inhibited cell viability. HGPDS and Flu co-cultured for 24 and 36 h restored cell viability. Fluorescence staining showed that HGPDS had a statistically significant difference in cell viability when treated with 1 × 10-6 mol / L Flu for 24 h (P <0.05). Compared with the normal control group, HGPDS significantly increased the expression of FN mRNA and protein in peritoneal mesothelial cells (P <0.05) in a time-dependent manner. The FN mRNA peaked at 6 h and the expression of FN protein Peak at 24 h. Flu inhibited HGPDS-induced high expression of FN (P <0.05) in a concentration-dependent manner. Conclusion: HGPDS induces the increase of FN expression in human peritoneal mesothelial cells in vitro, which can be inhibited by Flu.